| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequencein the middle region of human MYL6 was used as the immunogen for the MYL6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYL6 Antibody / Myosin light chain 6 is a anti-MYL6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: MYL6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
MYL6 is one of two major isoforms produced by alternative splicing: a smooth muscle form (MYL6) and a non-muscle form (MYL6B). The smooth muscle isoform plays a key role in regulating calcium-sensitive contraction via interaction with myosin light chain kinase (MLCK) and myosin phosphatase, both of which control phosphorylation of the myosin regulatory light chain. The balance between phosphorylated and dephosphorylated MYL6 modulates smooth muscle tone and cell movement. Its structural domains include EF-hand calcium-binding motifs that enable conformational changes during contractile activation.
Beyond its role in muscle contraction, MYL6 contributes to intracellular transport, cytokinetic ring formation, and endothelial barrier regulation. Alterations in myosin light chain phosphorylation have been linked to vascular diseases, asthma, and tumor metastasis. Overexpression or dysregulation of MYL6 has been observed in certain cancers and fibrotic disorders, reflecting its role in actomyosin tension and epithelial-to-mesenchymal transition (EMT). In cardiac tissue, MYL6 interacts with actin-associated proteins to influence sarcomere assembly and stress response signaling.
Immunohistochemical staining using MYL6 antibody shows cytoplasmic localization in smooth muscle fibers, endothelial cells, and myofibroblasts. This antibody is widely used in research involving muscle physiology, cytoskeletal organization, and cellular contractility. MYL6 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
