| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C25H27NO |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
N-Desmethyltamoxifen is the major metabolite of tamoxifen in humans. It is a weak antiestrogen but a ten-fold more potent inhibitor of protein kinase C (PKC) than tamoxifen. It also potently regulates ceramide metabolism in human AML cells, limiting ceramide glycosylation, hydrolysis, and sphingosine phosphorylation[1][2][3]. It is supplied as a white to off-white solid (C25H27NO, MW 357.50) at 99.59% purity.
Physical & Chemical Properties
| CAS Number | 31750-48-8 |
|---|---|
| Molecular Formula | C25H27NO |
| Molecular Weight | 357.50 g/mol |
| Purity | 99.59% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | CC/C(C1=CC=CC=C1)=C(C2=CC=C(OCCNC)C=C2)\C3=CC=CC=C3 |
| Target | PKC, Estrogen Receptor |
| Signaling Pathway | Epigenetics; TGF-beta/Smad; Vitamin D Related/Nuclear Receptor; Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 11.4 mg/mL (31.89 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 11.4 mg/mL (31.89 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
All seven glioma lines (T98G, U87, U138, U373, ALW, AUK, CAS cells) are strongly inhibited by N-desmethyltamoxifen (20-500 ng/ml; 48 hours)[1]. At 1.5-10 μM for 114 hours, N-desmethyltamoxifen inhibits growth of MCF 7 human mammary carcinoma cells[2]. N-desmethyltamoxifen is the major primary quantitative metabolite of tamoxifen, formed through CYP3A4/5-mediated catalysis[3].
Cell Viability Assay[2]
| Cell Line | MCF 7 human mammary carcinoma cells |
|---|---|
| Concentration | 1.5, 2.5, 5, 7.5, 10 μM |
| Incubation Time | 114 hours |
| Result | Inhibits growth of MCF 7 human mammary carcinoma cells |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Development and Application of an UPLC-MS/MS Method for Simultaneous Quantification of Abemaciclib and Tamoxifen with Their Active Metabolites in Rat Plasma: Application to a Pharmacokinetic Study. Pharmaceuticals (Basel) 2026 May 19;19(5):795. PMID: 42198468
