| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
N-MYC (MYCN) is a basic helix-loop-helix-leucine-zipper transcription factor of the MYC family. It heterodimerizes with its partner protein MAX and binds E-box DNA sequences to activate transcription of genes that drive cell growth, proliferation, ribosome biogenesis, and metabolism. During development, N-MYC is expressed in a lineage-specific manner and is essential for central nervous system formation and the proliferation of neural progenitor cells. Amplification or deregulated expression of MYCN is a well-established oncogenic event in aggressive childhood tumors, most notably neuroblastoma and medulloblastoma. Reporter systems with E-box response elements provide a quantitative readout of N-MYC transcriptional output for studies of development and cancer.
Product Description & Applications
The N-MYC Reporter Lentivirus is a transcription-factor reporter system that detects N-MYC-driven transcriptional activity in mammalian cells. The construct uses tandem consensus E-box motifs with optimized flanking sequences to read out N-MYC activity selectively, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines and flexible detection by microscopy, flow cytometry, or luminometry.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and are suited to difficult-to-transfect cells, supporting research on N-MYC in CNS development, lineage specification, and oncogenesis.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the N-MYC transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
