| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
N-MYC, encoded by MYCN, is a basic helix-loop-helix-leucine-zipper transcription factor of the MYC family. It heterodimerizes with MAX and binds E-box DNA elements to activate genes controlling cell growth, proliferation, ribosome biogenesis, and metabolism. During development, N-MYC drives proliferation of neural progenitor cells and is essential for central nervous system formation. Amplification of MYCN is a defining oncogenic event in high-risk neuroblastoma and is also found in medulloblastoma and other aggressive tumors. Studying wild-type N-MYC alongside point-mutant variants helps dissect how specific residues influence protein stability, transcriptional output, and oncogenic activity.
Product Description & Applications
This ORF cDNA lentivirus enables stable overexpression of human N-MYC (MYCN) and is available in both wild-type and P44L point-mutant forms, in which the substitution alters the protein at residue 44. Expression is driven by a CMV promoter, and each construct includes a C-terminal V5 tag fused to the N-MYC ORF, followed by a self-cleaving peptide and a GFP or RFP reporter; in marker-containing variants the selection marker lies downstream of the reporter, separated by a second self-cleaving peptide.
Delivered as a third-generation, VSV-G-pseudotyped system, the PEG- and sucrose-gradient-purified particles transduce a wide range of cells, including primary and thawed cultures, and support stable polyclonal lines established by antibiotic selection or FACS. The paired wild-type and mutant constructs support structure-function and oncogenesis studies of N-MYC.
About This Product
This ORF cDNA lentivirus enables stable overexpression of MYCN (NCBI Accession: NM_001293228) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
