| Field | Specification |
|---|---|
| Target | |
| Alternative names | NACA; HSD48; Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NACA around the phosphorylation site of Ser43. AA range:9-58 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016],
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes NACA (phospho Ser43). It is validated for Immunohistochemistry, Immunofluorescence, ELISA.
Validated Applications
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
|---|---|
| Immunohistochemistry | 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human NACA around the phosphorylation site of Ser43. AA range:9-58
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NACA around the phosphorylation site of Ser43. AA range:9-58 |
| Concentration | 1 mg/mL |
| Subcellular Location | Cytoplasm . Nucleus . The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus. . |
| Swiss-Prot | Q13765 |
| Gene ID | 4666 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for NACA (phospho Ser43) research from ELK Biotechnology, or contact us for alternative formats.
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