| Field | Specification |
|---|---|
| Target | |
| Alternative names | NANOGP8; Putative homeobox protein NANOGP8 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009],
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Nanog P8. It is validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry.
Validated Applications
| Applications | Western Blot, Immunofluorescence, ELISA, Immunohistochemistry |
|---|---|
| WB 1 | 500-2000 |
| IF/ICC 1 | 50-200 |
| ELISA 1 | 2000-20000 |
| IHC-p 1 | 50-200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100 |
| Concentration | 1 mg/mL |
| Observed Band | 35kD |
| Subcellular Location | Nucleus . |
| Swiss-Prot | Q6NSW7 |
| Gene ID | 388112 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Rat, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Nanog P8 research from ELK Biotechnology, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
