| Field | Specification |
|---|---|
| Formulation | |
| Product Type | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
Human core histones (two copies each of H2A, H2B, H3, and H4), purified from cultured HeLa cells. Histones are separated from DNA under high salt conditions.
Product Description
Mammalian (HeLa) ExpressionRecombinant Native Core Histone Octamers Human protein is produced using a validated Mammalian (HeLa) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| Expression System | Mammalian (HeLa) |
|---|---|
| Formulation | 50 mM sodium phosphate buffer, pH 6.8 and 2.5 M NaCl |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in Mammalian (HeLa). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
The expressed amino acid region is specified in the product description. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Ideal as a substrate for methylation or acetylation assays or for screening small molecule inhibitors of epigenetic enzymes in drug discovery and HTS applications. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
- Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.
- Zhang, Y., et al., Genes and Dev. 2001; 15: 2343-2360.
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