| Field | Specification |
|---|---|
| Species |
The NCI-H2126 cell line is derived from a human large cell carcinoma, a subtype of non-small cell lung cancer (NSCLC). Originating from the lung tissue of a male patient, this cell line exhibits characteristics typical of large cell carcinomas, including poorly differentiated, undifferentiated cellular features. It is an important model for understanding the genetic and molecular mechanisms underlying large cell lung cancers and for testing therapeutic agents targeting this NSCLC subtype.
Genomic studies on NCI-H2126 have identified frequent allelic losses and chromosomal aberrations, such as deletions on chromosome arms 6q and 13q, which are commonly implicated in tumor suppressor gene inactivation in NSCLC. These genetic alterations contribute to the disruption of key regulatory pathways, including those involved in cell cycle control and apoptosis. The cell line has been employed in comparative studies to distinguish patterns of chromosomal loss across different lung cancer subtypes, enhancing the understanding of NSCLC-specific molecular signatures.
NCI-H2126 has also been included in extensive drug screening programs to evaluate its sensitivity and resistance to various chemotherapeutic agents and targeted therapies. The cell line’s genetic profile and its tumorigenic potential in xenograft models make it a valuable resource for preclinical studies focused on the development and refinement of treatments for large cell carcinoma and other forms of NSCLC.
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- Isoenzymes: AK-1, 1, ES-D, 1-2, G6PD, B, GLO-I, 2, Me-2, 0, PGM1, 1-2, PGM3, 2
- Tumorigenic: Yes, in nude mice
- Viruses: EBV (Transformant)
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 5% FBS, 0.005 mg/mL Insulin, 0.01 mg/mL Transferrin, 30nM Sodium selenite, 10 nM Hydrocortisone, 10 nM beta-estradiol
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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