| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nec-5 |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C19H17N3O2S2 |
| Purity | |
| Activity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Necrostatin-5, also known as Nec-5, is a potent inhibitor of necroptosis with an EC50 of 0.24 μM. It also inhibits RIP1 and has been reported to show cardioprotective effects[1][2][3]. It is supplied as a white to off-white solid (C19H17N3O2S2, MW 383.49) at 99.40% purity.
Physical & Chemical Properties
| CAS Number | 337349-54-9 |
|---|---|
| Molecular Formula | C19H17N3O2S2 |
| Molecular Weight | 383.49 g/mol |
| Purity | 99.40% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | O=C(N1C2=CC=C(C=C2)OC)C3=C(N=C1SCC#N)SC4=C3CCCC4 |
| Target | RIPK1 |
| Signaling Pathway | Apoptosis |
| Bioactivity Class | necroptosis |
| Solubility | In Vitro: DMSO: 5 mg/mL (13.04 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
Activity & Target
[1][3]
|
necroptosis 0.24 μM (EC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 5 mg/mL (13.04 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
In a dose-dependent manner, Necrostatin-5 (1, 5, 10, 50, 100 μM) reduces LDH release[3]. At 100 μM, Necrostatin-5 shields MH-S cells from challenge with S. aureus, Listeria monocytogenes, S. pneumoniae, and uropathogenic Escherichia coli (UPEC), and also guards against pneumolysin-induced death[3].
In Vivo
In the isolated rat heart model, Necrostatin-5 (2.46 mg/kg; i.p.) shows cardioprotective effects[2].
| Animal Model | 200-300 g, male Wistar rats[2] |
|---|---|
| Dosage | 2.46 mg/kg |
| Administration | I.p.; 60 min before experiment |
| Result | Reduced the infarction zone caused by 30-min global ischemia and 120-min reperfusion. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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