nef Antibody

SKU:BHA10561602
Overview
Click light‑blue chips for details
nef-specific polyclonal antibody raised in Rabbit. Validated for ELISA, Western Blot in Human immunodeficiency virus 1 samples. Affinity-purified.
Format Unconjugated
Immunogen Recombinant
Applications 2 validated
Dilution WB:1:500-1:2000
Target Aliases 1 aliases
Options selector
Catalog no. Size
CSB-PA27307A0Rb-50UL 50 uL
CSB-PA27307A0Rb-100UL 100 uL
Available Options
Size
Field Specification
Mfr No CSB-PA27307A0Rb
Alternative Names Protein Nef, 3'ORF, Negative factor, F-protein, C-terminal core protein, nef
Antigen
  • Recombinant Human immunodeficiency virus 1 Protein Nef protein (1-209AA)
Clonality
  • Polyclonal
Conjugate
  • Non-conjugated
Form Liquid
Host Rabbit
Immunogen Recombinant Human immunodeficiency virus 1 Protein Nef protein (1-209AA)
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human immunodeficiency virus 1
Source This product is a polyclonal antibody purified from rabbit antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target nef
UniProt # A0A1L4CSJ1

Product Overview

Primary Antibody

This antibody targets nef and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA WB:1:500-1:2000
Western Blot (WB) WB:1:500-1:2000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target nef
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human immunodeficiency virus 1
Purification Affinity Purification
Antigen / Immunogen Recombinant Human immunodeficiency virus 1 Protein Nef protein (1-209AA)
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

Protein Nef, 3'ORF, Negative factor, F-protein, C-terminal core protein, nef

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
Targetnef
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman immunodeficiency virus 1
PurificationAffinity Purification
Antigen/ImmunogenRecombinant Human immunodeficiency virus 1 Protein Nef protein (1-209AA)
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage Buffer0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISAWB:1:500-1:2000
Western Blot (WB)WB:1:500-1:2000

Immunogen: Recombinant Human immunodeficiency virus 1 Protein Nef protein (1-209AA)

Antigen Species: Human immunodeficiency virus 1

Alternative Names

Protein Nef, 3'ORF, Negative factor, F-protein, C-terminal core protein, nef

What species does this nef antibody react with?
This antibody has been validated for reactivity against Human immunodeficiency virus 1. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Western Blot (WB). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:2000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Matches this product
15% OFF
15% Off Proteins from Trusted Suppliers — Limited Time
Limited Time PT15OFF
15%OFF
15% Off Cancer Antibodies
Ends Sep 30 ONCO15
$50 OFF
$50 Off All ELISA Kits
Limited time ELISA50
10% OFF
10% OFF CELL LINES-Limited-Time Offer
Ends Sep 30 CELL10
FREE SAMPLE
Free Sample – CellTrypase Recombinant Trypsin-Like Enzyme
Limited time offer – availa... FREESAMPLE
FREE SAMPLE
Free Sample – MycoFold™ Growth Factors
Limited time offer – availa... Request Free Sample

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today