| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Soluble in water. Centrifuge all product preparations before use (10000 x g, 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: (6R)-6-[(5S)-6,6-dimethyl-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-6-ium-5-yl]-6H-furo[3,4-g][1,3]benzodioxol-8-one;chloride.
- CAS number: 53552-05-9
- Molecular formula: C21H20ClNO6.
- Purity: >97% (HPLC)
- Concentration: 1-10 µM.
- Form: Lyophilized powder.
- Solubility: Soluble in water. Centrifuge all product preparations before use (10000 x g, 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: GABA(A) receptors
- Source: Synthetic
- Activity: (-)-Bicuculline methochloride is a water soluble quaternary salt of Bicuculline, a competitive antagonist of the GABAA receptors (IC50 = 8.4 µM)1. Quaternary salts of Bicuculline were shown to have effects not mediated by GABA receptors2.
Scientific background
(-)-Bicuculline methochloride is a synthetic compound that acts as water soluble GABA(A) receptor antagonist. The compound is a quaternary salt of bicuculline (BIC), a competitive antagonist of the GABA(A) receptors. (-)-Bicuculline methochloride is more stable at physiological pH and more soluble in aqueous solutions than bicuculline. The compound was shown to affect other neurotransmitter systems such as the cholinergic system1.GABA(A) receptors are members of the Cys-loop superfamily of ligand-gated ion channels. They mediate GABA (gamma-aminobutyric acid) action, a major inhibitory neurotransmitter, through the central nervous system (CNS). They play an important role in inhibiting cell excitation in the CNS. They are considered to be a major target of antiseizure drugs2,3.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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