| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Any aqueous buffer (up to 100mM stock). Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: (1R,5S)-1,2,3,4,5,6-hexahydro-1,5-methano-8H-pyrido[1,2-a][1,5]diazocin-8-one.
- Also known as: Baphitoxine, Sophorine
- CAS number: 485-35-8
- Molecular formula: C11H14N2O.
- Purity: >95%
- Concentration: 40 nM - 1 μM.
- Form: Lyophilized powder.
- Solubility: Any aqueous buffer (up to 100mM stock). Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Any aqueous buffer (up to 100mM stock). Centrifuge all product preparations before use (10000 x g 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: nAChR
- Source: Synthetic
- Activity: (-)-Cytisine is a potent and selective agonist of neuronal nAChR. It acts as a partial agonist at β2-containing nAChR1,2.
- Alternative names: Baphitoxine, Sophorine
Peptide confirmation
Confirmed by amino acid analysis and mass spectrometry
Scientific background
(-)-Cytisine is an alkaloid, which occurs naturally in several types of plants as well as in animals feeding on them (see example in reference 1). (-)-Cytisine is a potent and selective agonist of neuronal nAChR2. It acts as a partial agonist of β2-containing nAChR3.EC50s differ for different subunit-composed channels; for recombinant human channels expressed in Xenopus oocytes the following EC50 values were reported: 25 µM, 39 µM, 67 µM, 72 µM, 2.6 µM 0.9 µM and 71 µM for α2/β2, α2/β4, α3/β2, α3/β4, α4/β2, α4/β4 and α7 respectively4.However, in β2-containing channels, the response to acetylcholine (ACh) is largely reduced when applied in combination with (-)-Cytisine5. Therefore, when applied in vivo it exerts a compound effect of both activating certain nAchRs and inhibiting the effect of ACh on others. For example, (-)-Cytisine was found to have antidepressant-like effects in several animal models of antidepressant efficacy. This was probably achieved by inhibiting the effect of ACh on α2/β4 channel in the basolateral amygdala6.In another study, the effects of (-)-Cytisine were compared to nicotine. Rats were trained to discriminate nicotine from saline and it was confirmed that (-)-Cytisine had a nicotine-like discriminative effect, but it was much less potent than nicotine itself7.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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