| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human NELFB was used as the immunogen for the NELFB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NELFB Antibody / Negative elongation factor B / COBRA1 is an antibody targeting NELFB. Host: Rabbit. Monoclonal. Clone: 32C80. Format: Liquid. Applications: WB, IHC, ICC, IF.
Key elements and design rationale
- Target: NELFB.
- Immunogen: A synthesized peptide derived from human NELFB was used as the immunogen for the NELFB antibody..
- Species reactivity: Human.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
NELFB antibody targets negative elongation factor B, also known as COBRA1, a transcriptional regulator involved in RNA polymerase II pausing. NELFB functions as part of the NELF complex, which includes NELFA, NELFC/D, and NELFE subunits. This complex stabilizes paused RNA polymerase II at promoter-proximal regions, ensuring proper regulation of transcriptional elongation.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IHC: tissue distribution assessment; interpret with matched controls.
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
