Neuro-2a Cells

The Neuro-2a cell line, often abbreviated as N2A cells, is a mouse neuroblastoma cell line derived from the neural crest. These cells are known for their rapid proliferation and ability to differentiate into neuron-like cells under certain conditions, making them a valuable model for studying neurogenesis and neuronal differentiation. Neuro-2a cells exhibit characteristics typical of nerve cells or neuroblasts, which are precursors to fully differentiated neuronal cells. One of the key features of mouse Neuro 2a cells is their utility in exploring the mechanisms of differentiation, particularly in the context of dopaminergic neurons. These cells can be induced to express markers characteristic of dopamine neurons, including the dopamine transporter and proteins involved in dopamine receptor localization. This makes the N2A cell line an essential tool for studies related to the normal neuroendocrine system and disorders associated with dopaminergic signaling. The N2A cell line also provides insights into the role of various genes and proteins in neuronal function and development. For instance, the DNMT3A gene, known for its involvement in DNA methylation processes, has been studied in Neuro-2a cells to understand its impact on neuronal cells and neurodevelopmental processes. The expression of the human thyroid hormone receptor in these cells allows researchers to investigate thyroid hormone response and its influence on neurodevelopment and the differentiation of neuroblastoma cells into more mature neuronal phenotypes. Protein kinase signaling pathways are another area of intense study in N2A cells, given their critical role in mediating various cellular processes, including cell growth, differentiation, and response to extracellular signals. In summary, the Neuro-2a (N2A) cell line, derived from mouse neuroblastoma, serves as a versatile model for studying neurogenesis, neuronal differentiation, and dopaminergic signaling, providing valuable insights into the molecular underpinnings of neurodevelopmental processes and neuroendocrine disorders.

SKU:BHC11100445

• SDS (0345B32051364BCBAAA15847BCC6F20D)
• Product Sheet (0345B32051364BCBAAA15847BCC6F20D)
• Neuro-2a 400394-210324 Cytion CoA (PDF)

• Antigen expression: H-2a
• Viruses: Ectromelia virus (mousepox): negative
• Virus resistance: Poliovirus 1
• Reverse transcriptase: Negative
• Products: Tubulin, acetylcholinesterase

• cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
• supplements: Supplement the medium with 10% FBS and 1% NEAA
• dissociationReagent: Accutase
• subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
• seedingDensity: 1 x 104 cells/cm2
• fluidRenewal: 1 to 2 times per week
• postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
• freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.