| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
NeuroD1, also known as BETA2, is a basic helix-loop-helix transcription factor of the neural NeuroD family. It forms heterodimers with ubiquitous E-proteins and binds E-box elements to activate target genes including insulin, secretin, and pro-opiomelanocortin. NeuroD1 is expressed in neurons, the anterior pituitary, pancreatic islets, and enteroendocrine cells, where it directs neuronal differentiation, cerebral cortex development, and the maturation and expansion of insulin-producing pancreatic beta cells. At the secretin enhancer it cooperates synergistically with Sp1 and RREB1 to drive transcription. Because of its dual role in neural and endocrine programs, NeuroD1 is an important target in neuroscience, diabetes, and developmental biology research.
Product Description & Applications
The NeuroD1 Reporter Lentivirus measures NeuroD1 transcriptional activity in mammalian cells. It uses tandem repeats of NeuroD1 response elements taken from the secretin promoter enhancer, a context that reflects NeuroD1's synergistic interaction with Sp1 and RREB1, coupled to a minimal promoter driving a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.
Stable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on NeuroD1 in neuronal development and pancreatic beta-cell biology.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NeuroD1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
