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Neutravidin Plates are immunoassay plates coated with Neutravidin, specifically designed to bind biotinylated molecules with minimal non-specific binding. These plates are suitable for various applications, including binding biotinylated antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. Unlike Streptavidin, Neutravidin lacks the RYD sequence (Arg-Tyr-Asp), which eliminates interaction with the RGD sequence (Arg-Gly-Asp) found in cell membrane receptors, making it ideal for assays where cell receptor interference must be avoided. Neutravidin Coated 96-Well Plates are especially useful for molecules that do not reliably bind through passive adsorpt
Neutravidin Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Key Features
- Neutravidin surface: It binds biotinylated molecules, such as biotin-tagged antibodies.
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
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Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 50 to 100 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
Key Benefits
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Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
Applications
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Matson RS (2023). ELISA Essentials: Surfaces, Antibodies, Enzymes, and Substrates. Methods Mol Biol. 2612:1-18. PMID: 36795356.
- Bhatt M et al. (2016). Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay. PLoS One. 11(3):e0151154. PMID: 26954237.