| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
Pyroptosis is a pro-inflammatory form of programmed cell death triggered by inflammasome activation. When danger signals are sensed, the adaptor protein ASC polymerizes into a large cytoplasmic aggregate, the ASC speck, which serves as a platform for activation of caspase-1 (CASP1). Active caspase-1 cleaves the pore-forming protein gasdermin D (GSDMD) and proinflammatory cytokines, and the released GSDMD N-terminal fragment forms membrane pores that drive cell lysis and inflammation. The ASC speck is a hallmark of inflammasome assembly, and NF-κB signaling provides the priming step that upregulates inflammasome components, making ASC speck formation a valuable readout for studying pyroptosis and inflammation.
Product Description & Applications
The NFκB-ASC (Pyroptosis) Reporter Lentivirus is an immunotherapy reporter system designed to monitor pyroptotic ASC speck formation in response to inflammasome activation. The system expresses a fluorescently tagged ASC fusion protein, available as ASC-GFP or ASC-RFP, so that ASC polymerization can be visualized directly as discrete specks marking inflammasome complex assembly; additional reporter configurations include fluorescent and secreted Gaussia luciferase outputs.
Stably expressed in suitable cells such as THP-1 monocytes, the reporter enables in vitro study of pyroptosis, a pro-inflammatory form of programmed cell death linked to caspase-1 and gasdermin D activity. Supplied as VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product transduces difficult-to-transfect cells, including primary and thawed cultures, supporting inflammasome and inflammation research.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human Pyroptosis Assay under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (ASC-GFP Fusion Protein, ASC-RFP Fusion Protein, GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
