| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line |
| Product Type | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
NF-κB signaling plays a pivotal role in regulating cell development and immune homeostasis. Activation of NF-κB through tumor necrosis factor receptors (TNFR) or the TNFR superfamily member CD40 occurs upon engagement with their respective ligands TNFα or CD40L. Activation of NF-κB enhances cell inflammation and prevents apoptosis, which contribute to tumor development. The A549 lung epithelial cell line is ideal in an in vitro lung disease model for high throughput screening of oncogene inhibitors upstream of the NF-κB signaling pathway.
Product Description
Cell SignalingNF-κB luciferase reporter construct is stably integrated into the genome of A549 cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
Product Specifications
| Host Cell Line | A549 |
|---|---|
| Host Species | Human |
| Transfection Method | Lipofectamine |
| Format | Each vial contains ~3 x 106 cells in 1mL of 10% DMSO in FBS. |
| Harmonized Tariff Code | 3002-5900 |
Safety & Handling
⚠ Avoid freeze/thaw cycles.
Regulatory Information
License Disclosure
Related Products
Related Products: Cat. #72044, 71236, 60536, 60690, 79657, 79796
Required Accessories: Cat. #60183,60690,79657,79796
This product is engineered on a A549 background (Human origin). The A549 host was selected for its compatibility with stable transfection and the target pathway or assay type. Consult the product datasheet for passage number guidance and recommended culture media.
This product is classified as BSL-1. Standard microbiological practices (gloves, lab coat, eye protection) are sufficient. No specialized containment facility is required beyond a clean bench. Consult your institutional IBC for GMO registration requirements.
We recommend performing mycoplasma testing upon receipt and at regular intervals during culture, regardless of vendor QC status. Validated detection methods include PCR-based assays (e.g., Venor™GeM) and luminescence assays (e.g., MycoAlert™). Contamination can be introduced during routine handling.
Store this product at Liquid Nitrogen. Specifically: Store in liquid nitrogen immediately upon receipt. Transfer cells from dry-ice shipping to the recommended storage immediately upon receipt. Avoid repeated freeze-thaw cycles, which reduce viability and may alter expression characteristics.
Yes, a license is required (Yes). Purchase of this cell line grants a time-limited research-use license for use in your immediate laboratory only. This license does not permit redistribution, sub-licensing, transfer to other institutions, or commercial use. Refer to the License Disclosure section on this page or contact BPS Bioscience for details regarding modifications or commercial licensing.
This stable cell line was generated using Lipofectamine for transgene delivery into the parental host. The stably integrated cells were selected using the appropriate resistance marker and verified for expression prior to cryopreservation.
Can't find the cell line you need—or require a custom engineered model for your study? We offer end-to-end support for diverse research needs, including:
- Cell line sourcing and selection (species, tissue, and disease model matching)
- Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA)
- Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs)
- Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles)
- Inducible expression systems (Tet-On/Off and regulatable constructs)
- Drug resistance marker selection (puromycin, G418, hygromycin, and others)
- Custom growth and media optimisation for specific assay requirements
- Scale-up production for high-throughput screening campaigns
- Authentication and QC services (STR profiling, mycoplasma testing, viability assessment)
Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
- Cherfilis-Vicini J. et.al. (2010) Triggering of TLR7 and TLR8 expressed by human lung cancer cells induces cell survival and chemoresistance. J. Clin. Invest. 120: 1285-1297.
- Chen W. et.al. (2011NF-κB, a mediator for lung carcinogenesis and a target for lung cancer prevention and therapy. Front. Biosci. 16: 1172-1185.
- Callister ME et.al. (2008) PMX464, a thiol-reactive quinol and putative thioredoxin inhibitor, inhibits NF-κB-dependent proinflammatory activation of alveolar epithelial cells. Br. J. Pharm. 155: 661-672.
- Schmeck B et.al. (2007) Legionella pneumophilia-induced NF-κB- and MAPK-dependent cytokine release by lung epithelial cells. Eur. Respir. J. 29: 25-33
