| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
NF-κB is a family of transcription factors that coordinate immune responses, inflammation, cell survival, and proliferation. Active NF-κB complexes are dimers of Rel-family subunits; the p65 subunit, also called RelA, is a strong transcriptional activator. p65 most commonly partners with p50 in canonical signaling downstream of stimuli such as TNF-alpha, but it can also form p65/p65 homodimers with distinct transcriptional properties. Selective monitoring of p65 homodimer activity is useful for dissecting non-canonical aspects of NF-κB signaling, including responses in tumor cells that are separate from classical inflammatory activation. Dysregulated NF-κB signaling contributes to chronic inflammation, autoimmunity, and cancer, making it an important focus of immunology research.
Product Description & Applications
The NF-κB (p65) Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p65 (RelA) homodimer activity in mammalian cells. The construct contains tandem repeats of the p65 DNA-binding element placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. Used alongside a pan-NF-κB reporter, it allows discrimination of p65/p65 homodimer activity from p50/p65 heterodimer activity. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it transduces primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the NFκB (p65 homodimer) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
