| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
NFAT (nuclear factor of activated T cells) and GATA transcription factors cooperate to control cytokine gene expression in immune cells. The NFAT family (NFATc1-c4 and NFAT5) responds to calcium and calcineurin signaling downstream of antigen receptors and acts as an antigen-specific regulator of gene expression in T cells and myeloid cells. GATA3 is the master transcription factor of the Th2 lineage. At composite response elements, such as those in the IL-3 promoter, NFAT and GATA proteins bind together and synergistically activate Th2-associated cytokine genes including IL-4, IL-5, and IL-13. Reporters built on these composite elements provide a readout of NFAT/GATA co-regulated transcription central to allergic and adaptive immune responses.
Product Description & Applications
The NFAT/GATA Reporter Lentivirus is a transcription-factor reporter system for detecting synergistic NFAT and GATA transcriptional activity in mammalian cells. The construct uses tandem repeats of composite NFAT and GATA DNA-binding elements derived from the human IL-3 promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, so the readout reflects co-regulated activation by both factors. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on T-cell activation and Th2 cytokine regulation with readout by microscopy, flow cytometry, or luminometry.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NFAT + GATA Pathways transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
