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Product Overview
Nipah virus (NiV) is a zoonotic RNA virus belonging to the genus Henipavirus of the family Paramyxoviridae, together with Hendra virus. It was first identified in 1998 in Nipah village, Malaysia. Nipah virus infection can cause a range of clinical manifestations, including asymptomatic infection, acute respiratory disease, and fatal encephalitis, with an estimated mortality rate of 40–75%. The virus poses a significant threat to both human and animal health. Therefore, the establishment of rapid and accurate detection methods is of great importance for Nipah virus research.
This kit uses a fluorescent PCR (qPCR) method to detect the presence of Nipah virus in samples. The kit contains primers specifically designed for the conserved region of the NiV P protein, HotStarTaq DNA Polymerase, and an optimized qPCR buffer system, which together enhance amplification efficiency and enable effective amplification of low-concentration templates. Each 20 µL reaction is cycled at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec, followed by melting curve analysis.
Specifications
| Method | Fluorescent PCR (qPCR) |
|---|---|
| Target | Conserved region of the NiV P protein |
| Polymerase | HotStarTaq DNA Polymerase |
| Template | DNA; no reverse transcription step |
| Reaction volume | 20 µL |
| ROX | ROX Reference Dye supplied; volume varies by instrument |
| Controls included | NiV Positive Control |
| Reactions per kit | 50 rxn or 100 rxn |
Kit Components
| Component | 50 rxn | 100 rxn |
|---|---|---|
| 2× NiV qPCR Mix | 500 µL | 1 mL |
| Primer Mix | 50 µL | 100 µL |
| NiV Positive Control | 250 µL | 500 µL |
| ROX Reference Dye | 750 µL | 1.5 mL |
| RNase-free ddH₂O | 750 µL | 1.5 mL |
Applications
- Research-use detection of Nipah virus sequences in experimental samples
- Nipah virus research
Key Features
- Ready-to-use master mix; the user supplies the DNA template.
- Primers designed against a conserved region of the NiV P protein for high specificity; contact BioHippo for cross-reactivity data.
- NiV Positive Control included.
Storage & Handling
Stored at -20°C and protected from light, with a shelf life of one year. Shipping: Ice packs or dry ice.
Usage Notes
- Wear a lab coat and disposable gloves during the experimental procedures.
- Transport the kit with ice packs or dry ice.
- Avoid strong light exposure.
- Mix gently before use.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
