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Product Overview
Nipah virus (NiV) is a zoonotic RNA virus belonging to the genus Henipavirus within the family Paramyxoviridae, closely related to Hendra virus. It was first identified in 1998 in Nipah village, Malaysia. Nipah virus infection can lead to a wide range of clinical manifestations, including asymptomatic infection, acute respiratory infection, and fatal encephalitis, with an estimated mortality rate of 40% to 75%. Due to its significant impact on human and animal health, the establishment of rapid and accurate detection methods for Nipah virus is of great importance for related research.
This product utilizes the Taqman probe method to detect the presence of Nipah virus in samples. The kit contains primers and probes specifically designed for the conserved P protein region of NiV, HotStarTaq DNA Polymerase, and an optimized qPCR buffer system, which together enhance amplification efficiency and enable effective amplification of low-concentration templates.
Specifications
| Method | TaqMan probe qPCR |
|---|---|
| Target | Conserved P protein region of NiV |
| Polymerase | HotStarTaq DNA Polymerase |
| Template | DNA; no reverse transcription step |
| Reaction volume | 20 µL |
| ROX | ROX Reference Dye supplied; volume varies by instrument |
| Controls included | NiV Positive Control |
| Result principle | Qualitative; confirmed by the amplification curve |
| Reactions per kit | 50 rxn or 100 rxn |
Kit Components
| Component | 50 rxn | 100 rxn |
|---|---|---|
| 2× NiV qPCR Mix | 500 µL | 1 mL |
| Primer & Probe Mix | 50 µL | 100 µL |
| NiV Positive Control | 250 µL | 500 µL |
| ROX Reference Dye | 750 µL | 1.5 mL |
| RNase-free ddH₂O | 750 µL | 1.5 mL |
Applications
- Research-use detection of Nipah virus sequences in experimental samples
- Nipah virus research
Key Features
- Ready-to-use master mix; the user supplies the DNA template.
- Primers and probe designed against the conserved P protein region of NiV for high specificity; contact BioHippo for cross-reactivity data.
- Includes a NiV Positive Control for assay validation.
Storage & Handling
Stored at -20°C and protected from light, with a shelf life of one year. Shipping: Ice or dry ice.
Usage Notes
- Store at -20°C protected from light.
- Transport with ice or dry ice.
- Wear a lab coat and gloves during the procedure.
- Invert and mix thoroughly before use.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
