| Field | Specification |
|---|---|
| Target | |
| Alternative names | ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain family, member 15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; CD; CLR16.3; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; NLR family, CARD domain containing 2; NLRC2; NOD like receptor C2; NOD2; NOD2 protein; NOD2_HUMAN; NOD2B; nucleotide binding oligomerization domain 2; Nucleotide binding oligomerization domain containing 2; Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2; Nucleotide-binding oligomerization domain-containing protein 2; PSORAS1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide of Human NOD2 |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-NOD2 antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect NOD2 protein in Human and supports researchers working in cell biology contexts.
Key elements and design rationale
- Immunogen: Synthetic peptide of Human NOD2 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
NOD2 (also referred to as ACUG, Arthrocutaneouveal granulomatosis, BLAU, CARD15, Caspase recruitment domain family, member 15, Caspase recruitment domain protein 15, Caspase recruitment domain-containing protein 15, CD, CLR16.3, IBD1, Inflammatory bowel disease protein 1, LRR containing protein, NLR family, CARD domain containing 2, NLRC2, NOD like receptor C2, NOD2, NOD2 protein, NOD2_HUMAN, NOD2B, nucleotide binding oligomerization domain 2, Nucleotide binding oligomerization domain containing 2, Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2, Nucleotide-binding oligomerization domain-containing protein 2, PSORAS1) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of NOD2 biology in cell biology.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
NOD2 is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect NOD2 in ELISA, IHC applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known NOD2-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
