| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Noggin, symphalangism 1, SYM1, synostoses (multiple) syndrome 1, SYNS1 |
| Biological Activity | |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Reconstitution | |
| Shipping | |
| Species | |
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| UniProt # |
Scientific Background
Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
Product Description
Mammalian (HEK293) ExpressionRecombinant Noggin Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | Q13253 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 28-232(end) |
| Molecular Weight | 28 kDa |
| Endotoxin | <1 EU/µg (Noggin, by LAL method) |
| Formulation | Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2… |
| Storage | ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -2… |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 28-232(end). Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.
- Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233-2245.
- Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
