| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human NOLC1 was used as the immunogen for the NOLC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NOLC1 Antibody / Nucleolar and coiled-body phosphoprotein 1 / NOPP140 is a anti-NOLC1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30N31 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: NOLC1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30N31, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
NOLC1 antibody is widely applied in cell biology, cancer research, and nucleolar studies. By detecting NOLC1, researchers can investigate how the nucleolus coordinates ribosome production and stress responses. The protein is highly expressed in proliferating cells, linking it to growth and transformation. Dysregulated NOLC1 expression has been reported in cancers, where nucleolar function is often enhanced to meet elevated translational demands.
Applications of NOLC1 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated and unphosphorylated isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence highlights nucleolar localization and dynamics. These approaches provide powerful tools for studying nucleolar biology in health and disease.
NOLC1 interacts with RNA polymerase I transcription machinery, fibrillarin, and nucleolin, coordinating rRNA transcription and processing. It also functions in nucleolar stress responses, where its relocalization signals cellular stress or DNA damage. By applying NOLC1 antibody, scientists can study how nucleolar function integrates with signaling pathways in cancer, aging, and neurodegeneration.
NOLC1 mutations and expression changes contribute to oncogenesis. Overexpression correlates with tumor growth, while knockdown reduces proliferation. Because nucleolar size and activity are biomarkers of cancer, NOLC1 detection provides diagnostic and prognostic potential.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
