| Field | Specification |
|---|---|
| Analyte | |
| Analyte category | |
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Sample type(s) | Serum, Plasma, Cell Culture Supernatant |
| Detection method | |
| Detection instrument(s) | Flow Cytometer (Luminex-compatible or equivalent) |
| Instrument required | |
| Plate format | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Multi-Plex ( 2 Plex) Flow Cytometry Assay Panel - components to measure non-human Primate: Insulin-Like Growth Factor IGF-I and IGF-II simultaneously- in Primate Serum, Plasma, Cell Culture Supernatant or Cell Lysate samples.
Panel
Primate IGF-I/ IGF-II
Note
Universal Sample Diluent Kit is available for the Primate IGF-I IGF-II panel ( Universal for all sample types) Order cat# DKIGF2 (Diluent Kit) Cell Lysate Prep Buffer (CLP200) or Sample Dilution Buffer ( to dilute high concentration samples) -(SDL200) also available. (STARTER Kit Included)
Kit Contents
- Antibody Conjugated Beads
- Detection Antibody - Biotin
- Detection Antibody Diluent
- Standard: One vial of lyophilized standard containing
- 5.0 mL (1X) (Pre-Mixed , 2-Plex)
- 1.5 mL (2X) (Pre-Mixed , 2-Plex)
- 1.5 mL (2X)
- Pre-mixed multiplexed analytes ( 2-Plex) ( see vial insert) -
- single standard vial can be used for one or more assays indicated.
STARTER Kit Included
- Antibody Conjugated Beads
- Detection Antibody - Biotin
- Detection Antibody Diluent
- Standard: One vial of lyophilized standard containing
- 5.0 mL (1X) (Pre-Mixed , 2-Plex)
- 1.5 mL (2X) (Pre-Mixed , 2-Plex)
- 1.5 mL (2X)
- Pre-mixed multiplexed analytes ( 2-Plex) ( see vial insert) -
- single standard vial can be used for one or more assays indicated.
- Filter plate with lid Plate Sealers
- Streptavidin-PE
- PCR 8 tube strip
- 5.0 mL (10X)
- 20.0 mL (10X) ( 96 wells) (6)
- 3.0 mL (1X) 2 strips
- Sample Prep: Note: Serum and Plasma samples, and other sample types may
- need pre-treatment to release IGF-I and IGF-II for accurate
- measurement. An acid-ethanol extraction method described by: Breier,
- et al, ( J. Endocrinology; 1991; V128; 347-357) may be useful.
- Appropriate pre-treatment method for sample type and experimental
- conditions should be determined by investigator.
Assay Performance
- Sample Volume: 15.0 uL per test
- Cross-Reactivity: within panel : @ 10,000 pg/mL
- Intra-Assay CV: <10%
- Inter-Assay CV: <20%
- Recovery: 70-130%
Storage
Store Refrigerated ( 4-8°C. - in dark)
⚠ Caution
Contains Sodium Azide - ( 0.05%) - Dispose contents
| Parameter | Value |
|---|---|
| Sample Volume | 15.0 µL per test |
| Intra-Assay CV | <10% |
| Inter-Assay CV | <20% |
| Recovery | 70–130% |
| Cross-Reactivity (within panel) | Negligible |
| Approximate Assay Time | ~3 Hours |
Performance data sourced from Antigenix America product datasheet. Actual values may vary by lot. Refer to certificate of analysis supplied with kit.
What sample types are compatible?
This Human Super-X Plex™ 2-plex assay is compatible with serum, plasma, cell culture supernatant, and cell lysate samples. A species-matched Sample Diluent Kit (DKH100 (Human/Primate Serum/Plasma)) must be ordered separately.
What instrument is required?
A flow cytometer capable of detecting PE fluorescence is required. The assay is compatible with Luminex-based platforms and standard flow cytometers with bead-based multiplex detection capability.
What analytes are included in this panel?
IGF-1, IGF-II.
How much sample is needed per test?
15 µL per test. 96 Tests kit provides sufficient reagents for a full standard curve plus samples.
Looking for a specific analyte, species, or panel configuration not listed? The Super-X Plex™ platform is designed for flexibility — from off-the-shelf single-plex assays to fully configured custom multi-plex panels and bespoke kit builds.
Custom Multi-Plex Panel Design
Individual Super-X Plex™ assays within the same species group can be combined into custom multiplex configurations without additional cross-reactivity validation. Select any combination of analytes from the available group catalog — from 2-plex to 24-plex — and receive a single pre-mixed standard and matched bead set. Custom panels are available for Human, Mouse, Rat, Canine, and Non-Human Primate matrices.
Custom Kit Services
Beyond panel configuration, BioHippo and Antigenix America offer extended custom kit services to support specialized study designs:
- Analyte selection: Access to the full Super-X Plex™ catalog, including analytes not listed online. If your target is not shown, contact us — additional cytokines, chemokines, growth factors, and soluble receptors may be available.
- Species expansion: Custom assay development for species beyond the standard catalog (Equine, Feline, Porcine, Bovine, and others) may be available upon inquiry.
- Sample matrix compatibility: Assays are validated for serum, plasma, cell culture supernatant, and cell lysate. Custom matrix validation or adjusted diluent formulations can be explored for non-standard sample types.
- Kit size and format: Standard kits are supplied as 96-test formats. Smaller pilot formats (32-test) are available for select panels. Bulk quantities and custom packaging for high-throughput or multi-site studies can be quoted on request.
- Pre-mixed standard panels: Custom pre-mixed lyophilized standards can be formulated to match your exact panel composition, simplifying standard curve preparation across multiple assay runs.
- White-label and OEM supply: For core facilities, CROs, or institutional programs requiring custom-labeled or privately packaged kits, OEM supply arrangements can be discussed.
How to Request
Click Talk to a Scientist to submit a customization request form, email us at support@biohippo.com, or explore our Research Services for additional support options. Our team will review your requirements and respond promptly.
