| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Non-structural polyprotein (Polyprotein nsP1234) (P1234) [Cleaved into: P123, P123', mRNA-capping enzyme nsP1 (EC 2.1.1.-) (EC 2.7.7.-) (Non-structural protein 1), Protease nsP2 (EC 3.1.3.33) (EC 3.4.22.-) (EC 3.6.1.15) (EC 3.6.4.13) (Non-structural protein 2) (nsP2), Non-structural protein 3 (nsP3), Non-structural protein 3' (nsP3'), RNA-directed RNA polymerase nsP4 (EC 2.7.7.48) (Non-structural protein 4) (nsP4)] |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Venezuelan equine encephalitis virus (strain Trinidad donkey) (VEEV) Non-structural polyprotein (27-167aa) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-Non-structural polyprotein antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect Non-structural polyprotein protein in Venezuelan equine encephalitis virus and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Venezuelan equine encephalitis virus (strain Trinidad donkey) (VEEV) Non-structural polyprotein (27-167aa) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
Non-structural polyprotein (also referred to as Non-structural polyprotein (Polyprotein nsP1234) (P1234) [Cleaved into: P123, P123', mRNA-capping enzyme nsP1 (EC 2.1.1.-) (EC 2.7.7.-) (Non-structural protein 1), Protease nsP2 (EC 3.1.3.33) (EC 3.4.22.-) (EC 3.6.1.15) (EC 3.6.4.13) (Non-structural protein 2) (nsP2), Non-structural protein 3 (nsP3), Non-structural protein 3' (nsP3'), RNA-directed RNA polymerase nsP4 (EC 2.7.7.48) (Non-structural protein 4) (nsP4)]) is a protein target studied in Venezuelan equine encephalitis virus (strain Trinidad donkey) (VEEV) systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of Non-structural polyprotein biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Venezuelan equine encephalitis virus. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Non-structural polyprotein is a protein target in Venezuelan equine encephalitis virus biology. This polyclonal antibody raised in Rabbit is designed to detect Non-structural polyprotein in ELISA, WB applications, with IgG isotype.
Reported reactive against Venezuelan equine encephalitis virus. Immunogen derived from Venezuelan equine encephalitis virus (strain Trinidad donkey) (VEEV). Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known Non-structural polyprotein-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
