| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PBMC, primary cells |
| Product Type | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
Human peripheral blood mononuclear cells (PBMCs) include all the cells present in the peripheral blood that have a round nucleus. It is thus a mix of lymphocytes, such as T, B and NK cells, and monocytes. Most cells present in PBMCs are lymphocytes, with T cells being the main cell type. The proportion of each cell type present can be determined by using cell specific marker combinations, for instance NK cells are characterized as CD3-CD56+ cells. The use of these markers also allows for isolation of cell populations of interest. PBMCs are a critical and widely used cell source in the immunology, infectious disease, vaccine, cancer therapy and transplant immunology fields.
Product Description
Immune Cell MarkersCryopreserved vial (100 x 106, 30 x 106, 10 x 106 or 2 x 106 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis /apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.
Product Specifications
| Harmonized Tariff Code | 3002-5900 |
|---|
Usage Notes
Prepare a 50 ml conical tube with 10 ml of pre-warmed medium (10% FBS in IMDM or LGM). Quickly thaw cells in a 37°C water bath with constant and slow agitation. It is important to work quickly in the following steps to ensure high cell viability and recovery. Clean the outside of the vial with 70% ethanol and immediately transfer the entire content of the tube into the medium. Gently swirl the tube and centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in 15-20 mL of warm medium. Centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in warm medium and mix by gently flicking the tube. NOTE: Up to 30% of cell loss can be expected during washing steps. Cells are now ready for use in downstream applications.
Safety & Handling
⚠ Avoid freeze/thaw cycles.
Regulatory Information
License Disclosure
Related Products
Related Products: Cat. #79752, 79753
This product is classified as BSL-1. Standard microbiological practices (gloves, lab coat, eye protection) are sufficient. No specialized containment facility is required beyond a clean bench. Consult your institutional IBC for GMO registration requirements.
We recommend performing mycoplasma testing upon receipt and at regular intervals during culture, regardless of vendor QC status. Validated detection methods include PCR-based assays (e.g., Venor™GeM) and luminescence assays (e.g., MycoAlert™). Contamination can be introduced during routine handling.
Store this product at Liquid Nitrogen. Specifically: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -135°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival. Transfer cells from dry-ice shipping to the recommended storage immediately upon receipt. Avoid repeated freeze-thaw cycles, which reduce viability and may alter expression characteristics.
A standard research-use license applies (No). Commercial applications or transfer to other facilities may require a separate license. Contact BPS Bioscience for details.
Can't find the cell line you need—or require a custom engineered model for your study? We offer end-to-end support for diverse research needs, including:
- Cell line sourcing and selection (species, tissue, and disease model matching)
- Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA)
- Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs)
- Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles)
- Inducible expression systems (Tet-On/Off and regulatable constructs)
- Drug resistance marker selection (puromycin, G418, hygromycin, and others)
- Custom growth and media optimisation for specific assay requirements
- Scale-up production for high-throughput screening campaigns
- Authentication and QC services (STR profiling, mycoplasma testing, viability assessment)
Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
