| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The Notch signaling pathway is an evolutionarily conserved system of cell-to-cell communication that governs cell fate decisions, differentiation, proliferation, and tissue patterning. Ligand binding to Notch receptors triggers sequential proteolytic cleavages that release the Notch intracellular domain (NICD). NICD translocates to the nucleus and forms a transcriptional complex with the DNA-binding factor CSL (RBPJ) and Mastermind co-activators, activating target genes such as those of the HES and HEY families. Notch signaling is essential in development, stem cell maintenance, and immune cell differentiation, and its aberrant activation or loss contributes to cancers including T-cell acute lymphoblastic leukemia, as well as to vascular and developmental disorders.
Product Description & Applications
The NOTCH Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent or luminescent readout in response to Notch signaling pathway activity in human and mouse cells. Notch-responsive elements drive expression of the selected reporter, giving a quantitative measure of NICD-CSL-mediated transcription. The particles are purified by PEG precipitation and sucrose gradient centrifugation and are ideal for studying Notch activity in difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed drug-selection marker (puromycin or blasticidin) supports establishment of stable reporter cell lines, and the wide choice of reporters supports readout by fluorescence microscopy, flow cytometry, or luminometry for developmental biology, stem cell, and pathway-modulator studies.
About This Product
This reporter lentivirus places a d2GFP, Firefly Luc, GFP, GFP-P2A-FLuc, Luc, Luc+GFP, Renilla Luc, RFP, RFP-P2A-FLuc reporter gene under the control of tandem consensus response elements specific for the Notch signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
