| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
NPAS4 (neuronal PAS domain protein 4) is an activity-dependent, calcium-regulated transcription factor expressed predominantly in the brain. Induced rapidly by neuronal activity and calcium influx, NPAS4 functions as an immediate-early gene that orchestrates the development and refinement of neural circuits. It is a key regulator of the balance between excitatory and inhibitory synaptic inputs, controlling downstream targets such as Bdnf, Nptx2, and Frmpd3 that shape synaptic plasticity. By tuning excitatory-inhibitory balance, NPAS4 contributes to learning, memory, and protection against hyperexcitability disorders such as epilepsy, making it a central factor in neuroscience research.
Product Description & Applications
The NPAS4 Reporter Lentivirus is a transcription-factor reporter system for robust detection of human and mouse NPAS4 activity. It contains tandem repeats of NPAS4 response elements, based on the central midline element, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary neurons and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study activity-dependent NPAS4 transcription in neuroscience research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NPAS4 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
