| Field | Specification |
|---|---|
| Cellosaurus | CVCL_4396 |
| Species | |
| Applications | |
| Classification | |
| Tissue | |
| Age | |
| Sex | |
| Growth properties | |
| Biosafety level | |
| Catalog no. (Mfr.) | |
| Main SKU |
The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.Subsequently the requirement for exogenous growth factors was lost.The cells exhibit characteristics of macrophage cells.Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.NR8383 cells respond to bleomycin by secreting latent transforming growth factor(TGF beta).Stimulation with bleomycin also increases TGF beta mRNA expression.These cells are sensitive to endotoxin.LPS levels of 1 to 10 ng/mL inhibit replication by 50%.LPS inhibition is nontoxic and reversible even after levels up to 0.001mg/mL for extended periods.
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- Receptors expressed: Fc
- Protein expression: Transforming Growth Factor Beta(Tgf Beta), Interleukin 1(Il-1), Interleukin 6(Il-6)
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 15% heat-inactivated FBS
- dissociationReagent: Accutase, only of the adherent cells, floating cells must be collected separately.
- subculturing: Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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