NRF2 polyclonal antibody

SKU:BHA11304664
Overview
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Anti-NRF2 antibody (Rabbit). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Rat, Pig targets.
Host Species Rabbit
Species Reactivity Human, Mouse, Rat, Pig
Conjugate Unconjugated
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Method Affinity Purification
Options selector
Catalog no. Size
BS6286-50UL 50 uL
BS6286-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BS6286
Alternative Names NFE2L2;HEBP1;NRF2
Conjugate
  • Unconjugated
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Host Rabbit
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NRF2.
Molecular Weight ~ 110 kDa
Product Type
  • Primary Antibody
Purity > 95% (by SDS-PAGE)
Reactivity
  • Human
  • Mouse
  • Rat
  • Pig
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Overview

Research Use Only

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Validated Applications

Western Blot (WB) 500 - 1:1000|IHC
Immunohistochemistry (IHC) 50 - 1:200|IF/ICC
Immunofluorescence (IF/ICC) 100 - 1:500

Key Specifications

Host Species Rabbit
Species Reactivity Human, Mouse, Rat, Pig
Conjugate Unconjugated
Purification Affinity Purification
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Molecular Weight ~ 110 kDa
Catalog No. BS6286
UniProt Q16236
Alternative Names NFE2L2;HEBP1;NRF2

Immunogen / Antigen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NRF2.

Reactivity Notes

NRF2 polyclonal antibody detects endogenous levels of NRF2 protein.

Storage & Handling

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the NRF2 polyclonal antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:100 - 1:500

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human, Mouse, Rat, Pig. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NRF2.

How should this antibody be stored and handled?

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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