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The NRK cell line, derived from a Rattus norvegicus (rat) kidney, is an invaluable tool in biological research. These cells possess an epithelial morphology, meaning they form sheets covering the organs' surfaces and protecting against foreign substances.Epithelial cells, like NRK cells, exhibit specific characteristics. They have a generous amount of cytoplasm and contain numerous granules. These cells serve various bodily functions, with some acting as absorptive or protective agents while others act primarily as secretory cells.
In the case of the kidneys, the epithelial cells play a crucial role in the storage and subsequent secretion of excretory materials. This makes the NRK cell line particularly suitable for studying renal physiology. By utilizing these cells, researchers can investigate the intricate processes involved in kidney function and gain insights into various aspects of renal physiology.
Moreover, the NRK cell line is not limited to studying renal physiology alone. These versatile cells can also be employed in cancer research. Their epithelial morphology and origin from a normal rat kidney make them an excellent model for investigating the behaviour and characteristics of cancer cells in a controlled environment.
One application that leverages the unique properties of NRK cells is 3D cell culture. This technique involves growing cells in a three-dimensional matrix miming the natural cellular environment more closely than traditional two-dimensional culture. NRK cells can be cultured in this manner, allowing researchers to create complex tissue models that closely resemble the native structure of the kidney. This facilitates the study of cellular behaviour, interactions, and responses in a more physiologically relevant context.The NRK cell line is a valuable resource in biological research, specifically in cancer and renal physiology. These epithelial cells, derived from the kidney of an average rat, offer researchers the opportunity to delve into the intricacies of renal function and study cancer cells in a controlled laboratory setting. With their applicability in 3D cell culture, NRK cells enable the creation of realistic tissue models for comprehensive investigations into cellular behaviour and responses.
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- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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