| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Source | Synthetic |
| Molecular formula | C8H4Cl2N2O2. |
| Purity | |
| Activity | |
| Reconstitution | |
| Solubility | DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Concentration | |
| Form | Lyophilized powder. |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product details
- Chemical name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime.
- CAS number: 18711-16-5
- Molecular formula: C8H4Cl2N2O2.
- Purity: >99% (HPLC)
- Concentration: 0.1 - 10 µM.
- Form: Lyophilized powder.
- Solubility: DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: KCa2 and KCa3.1 K+ channels
- Source: Synthetic
- Activity: NS-309 is a positive modulator of small- and intermediate- conductance Ca2+-activated K+ (KCa2 and KCa3.1) channels, with EC50 values of 30 and 10 nM, respectively1. NS-309 also blocks L-type Ca2+ channels with IC50 value of 10 μM2.
Scientific background
Ca2+-activated potassium channels (KCa) are a group of 6/7-TM proteins that selectively transport K+ ions across biological membranes. They are broadly classified into three subtypes: SK, IK and BK channels (small, intermediate and big conductance, respectively). Three SK channel subtypes exist (SK1-3, KCa2.1-3)1 and are widely distributed in the central nervous system (CNS)1-2 as well as in the periphery. They constitute a molecular family of voltage-independent channels with the peripherally expressed intermediate conductance Ca2+-activated K+ channel (IK, KCa3.1)3, but differ in their distribution; SK channels are predominantly expressed in excitable cells, whereas IK channels mostly are found in non-excitable cells3-4.NS-309 is a selective and potent activator of KCa3.1 and KCa2 channels, whereas it is devoid of effect on BK (KCa1.1) type channels5-6. Whole cell currents, obtained by voltage ramps applied to HEK293 cells, reveal that human KCa2 channels are two to four times less sensitive to NS-309 than human KCa3.1; NS-309 increases the KCa2 currents with an EC50 value of 30 nM, whereas an EC50 value of 10 nM is inferred for the KCa3.1 currents5.NS-309 possesses a therapeutic potency for cystic fibrosis and chronic obstructive pulmonary disease by improving epithelial Cl- secretion via KCa3.1 channel activation7 and for moderating neuronal hyperexcitability by enhancing apamin-sensitive after-hyperpolarizing current (IAHP, mediated by KCa2 channels)5-6.NS-309 shows substantial block of L-type Ca2+ channel. It concentration-dependently inhibits the ICa in smooth muscle cells isolated from mouse urinary bladder with an IC50 value of 10.6 mM8. However, the specificity of NS-309 for the KCa3.1 channel over VDCC is much higher.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
