| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime.
- CAS number: 18711-16-5
- Molecular formula: C8H4Cl2N2O2.
- Purity: >99% (HPLC)
- Concentration: 0.1 - 10 µM.
- Form: Lyophilized powder.
- Solubility: DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: KCa2 and KCa3.1 K+ channels
- Source: Synthetic
- Activity: NS-309 is a positive modulator of small- and intermediate- conductance Ca2+-activated K+ (KCa2 and KCa3.1) channels, with EC50 values of 30 and 10 nM, respectively1. NS-309 also blocks L-type Ca2+ channels with IC50 value of 10 μM2.
Scientific background
Ca2+-activated potassium channels (KCa) are a group of 6/7-TM proteins that selectively transport K+ ions across biological membranes. They are broadly classified into three subtypes: SK, IK and BK channels (small, intermediate and big conductance, respectively). Three SK channel subtypes exist (SK1-3, KCa2.1-3)1 and are widely distributed in the central nervous system (CNS)1-2 as well as in the periphery. They constitute a molecular family of voltage-independent channels with the peripherally expressed intermediate conductance Ca2+-activated K+ channel (IK, KCa3.1)3, but differ in their distribution; SK channels are predominantly expressed in excitable cells, whereas IK channels mostly are found in non-excitable cells3-4.NS-309 is a selective and potent activator of KCa3.1 and KCa2 channels, whereas it is devoid of effect on BK (KCa1.1) type channels5-6. Whole cell currents, obtained by voltage ramps applied to HEK293 cells, reveal that human KCa2 channels are two to four times less sensitive to NS-309 than human KCa3.1; NS-309 increases the KCa2 currents with an EC50 value of 30 nM, whereas an EC50 value of 10 nM is inferred for the KCa3.1 currents5.NS-309 possesses a therapeutic potency for cystic fibrosis and chronic obstructive pulmonary disease by improving epithelial Cl- secretion via KCa3.1 channel activation7 and for moderating neuronal hyperexcitability by enhancing apamin-sensitive after-hyperpolarizing current (IAHP, mediated by KCa2 channels)5-6.NS-309 shows substantial block of L-type Ca2+ channel. It concentration-dependently inhibits the ICa in smooth muscle cells isolated from mouse urinary bladder with an IC50 value of 10.6 mM8. However, the specificity of NS-309 for the KCa3.1 channel over VDCC is much higher.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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