NS0 cell

SKU:BHC11101700
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Overview
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NS0 cell is a Lymphoblastoid cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Mouse multiple myeloma
Growth Properties Suspension
Tissue Plasma cell myeloma, hybridoma fusion partner
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Catalog no. Size
400109 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 400109
Species Mouse
NS0 is a murine myeloma cell line derived from a non-secreting variant of a mouse plasmacytoma. It is widely used in biotechnology and pharmaceutical manufacturing for the production of recombinant monoclonal antibodies and other therapeutic proteins. NS0 cells are adapted for suspension culture and can grow in serum-free, chemically defined media, making them well-suited for large-scale bioprocessing under current good manufacturing practice (cGMP) conditions. They are known for their high transfection efficiency and ability to achieve high protein expression yields, particularly when used in conjunction with strong mammalian expression vectors and amplification systems such as those based on methotrexate (MTX) selection. Despite their utility in protein production, NS0 cells are of murine origin, which introduces some limitations, including the presence of non-human glycosylation patterns on expressed proteins. These differences can influence immunogenicity and pharmacokinetics, which is a consideration in clinical applications. Nonetheless, NS0-derived products have received regulatory approval and are in clinical use, underscoring the line’s robustness and scalability. NS0 cells are non-tumorigenic and lack endogenous immunoglobulin expression, reducing the risk of contaminating native antibody sequences in recombinant antibody production workflows.

SKU:BHC11101700

Mutational profile:

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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