| Field | Specification |
|---|---|
| Alternative names | REDD1 inducer-1 |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C23H26N2O4 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
NS1-IN-1, also known as REDD1 inducer-1, is a naphthalimide compound and an NS1 inhibitor. It antagonizes influenza A virus NS1-mediated inhibition of host gene expression, induces REDD1 expression, and suppresses the mTORC1 signaling pathway via a TSC1-TSC2-dependent mechanism. It exhibits broad antiviral activity against influenza virus and vesicular stomatitis virus (VSV), with low toxicity[1]. It has the molecular formula C23H26N2O4 and a molecular weight of 394.46 g/mol.
Physical & Chemical Properties
| CAS Number | 181373-35-3 |
|---|---|
| Molecular Formula | C23H26N2O4 |
| Molecular Weight | 394.46 g/mol |
| SMILES | O=C(O)CCCCCN(C(C1=CC=C(N2CCCCC2)C3=CC=CC4=C13)=O)C4=O |
| Signaling Pathway | Anti-infection; PI3K/Akt/mTOR |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
NS1-IN-1 (25-50 μM; 24-30 h) decreases replication of influenza virus and expression of viral proteins (NP, M1, etc.) in MDCK cells while showing low cytotoxicity, in line with a cell viability assay giving CC50/IC50 ≈ 31[1]. In A549 cells, NS1-IN-1 (30 μM; 4-9 h) inhibits influenza viral protein expression and induces REDD1, while suppressing mTORC1 signaling through a TSC1-TSC2 dependent mechanism[1]. NS1-IN-1 (30 μM; up to 18 h) induces REDD1 mRNA transcriptionally without activating IFN-β or ISGs, confirming an interferon-independent antiviral mechanism[1]. NS1-IN-1 (25-50 μM; 24 h) additionally blocks VSV infection in MDCK cells, with antiviral activity depends on the REDD1-TSC1-TSC2-mTORC1 axis[1].
Cell Viability Assay[1]
| Cell Line | MDCK, MEF, HBEC cells |
|---|---|
| Concentration | 0.2-100 μM |
| Incubation Time | 24-72 h |
| Result | Showed low cytotoxicity in host cells, with CC50/IC50 selectivity ratio of approximately 31. |
Western Blot Analysis[1]
| Cell Line | MDCK, A549 cells |
|---|---|
| Concentration | 30 μM |
| Incubation Time | 4-9 h post-infection |
| Result | Downregulated influenza viral proteins (NS1, NP, M1, etc.) and induced REDD1 protein (6-8 fold). Reduced S6K phosphorylation at Thr389 (mTORC1 inhibition) without directly affecting AKT in early stages. |
Real Time qPCR[1]
| Cell Line | A549 cell |
|---|---|
| Concentration | 30 μM |
| Incubation Time | Various time points (up to 18 h pretreatment + infection) |
| Result | Transcriptionally induced REDD1 mRNA expression; induction was abolished by actinomycin D. Did not induce IFN-β or interferon-stimulated genes. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Microglial SIRT1 activation attenuates synapse loss in retinal inner plexiform layer via mTORC1 inhibition. J Neuroinflammation 2023 Sep 5;20(1):202. PMID: 37670386
