Nucleic Acid Binding Blocker

SKU:BHT16300175
Overview
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Blocker for ProFoldin 96-well DNA-binding and protein-binding plates when the binding partner is a nucleic acid. Supplied as 0.5 ml of 200 x stock, diluted 200-fold in low-salt buffer before use.
Dilution 200-fold
Concentration 200 x
Shipping Room Temperature
Options selector
Catalog no. Size
NBP96N 0.5 ml (200 x)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 0.5 ml (200 x) – NBP96N
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Stable at room temperature; store at 4°C or below.
  • Shipping: Shipped at room temperature.
  • Upon receipt: unpack promptly and store as described above.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Protein Interaction
Concentration 200 x
Storage Stable at room temperature; store at 4°C or below.
Shipping Shipped at room temperature.
Catalog no. (Mfr.) NBP96N
Main SKU BHT16300175
Blocking Reagents

Product Overview

The Nucleic Acid Binding Blocker blocks non-specific binding on ProFoldin 96-well DNA-binding and protein-binding plates when the second molecule in a binding experiment is a nucleic acid. It is supplied as a 200 x stock that is diluted 200-fold in low-salt buffer before use.

In the plate protocols, the wells are blocked with 1 x blocker in low-salt buffer after the first molecule has been bound, and before the nucleic acid partner is added.

Product Description

0.5 ml (200 x) – NBP96N 0.5 ml of 200 x concentrated blocker

Specifications

Concentration 200 x stock
Volume 0.5 ml
Dilution 200-fold in low-salt buffer before use
Use with 96-Well DNA-Binding Plates and Protein-Binding Plates
Catalog # NBP96N
Storage Stable at room temperature; store at 4°C or below.
Shipping Shipped at room temperature.

The ProFoldin protocols “96-well DNA–binding plates” and “Protein-binding Plates” are linked under Documents.

Notes

  • Catalog # NBP96N is this blocker solution. Catalog #s NBP96B (black) and NBP96T (transparent) are the 96-well DNA-binding plates it is used with; it is also used with the 96-well protein-binding plates (APP96B/T, CPP96B/T).

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.
Q.What is supplied?
A.0.5 ml of 200 x concentrated Nucleic Acid Binding Blocker (catalog # NBP96N).
Q.When should I use it?
A.Use it to block the binding plates when the second molecule is a nucleic acid. When the second molecule is a protein, the plate protocols use 1% BSA instead.
Q.How do I prepare the working solution?
A.Dilute the 200 x stock 200-fold in low-salt buffer (for example 10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) before use.
Q.Is NBP96N a plate?
A.No. NBP96N is the 200 x blocker solution. The 96-well DNA-binding plates are catalog #s NBP96B (black) and NBP96T (transparent).
Q.Is a protocol available?
A.Yes. The ProFoldin protocols “96-well DNA–binding plates” and “Protein-binding Plates” are linked under Documents.
Q.How is it shipped and stored?
A.Shipped at room temperature. Stable at room temperature; store at 4°C or below.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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