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Product Overview
Nucleic acids interfere with many steps of protein work, including cell lysis, chromatographic purification, protein quantification, activity assays and crystallization, especially when the protein of interest binds DNA or RNA. The Nucleic Acid Removal Kit removes nucleic acids from a protein solution or cell lysate to below 250 pg/ml.
NAR Reagent 1 and Reagent 2 are ionic polymers that precipitate nucleic acids, which are then removed by centrifugation. The final solution contains 0.45 M NaCl, and the reagents are easily removed during subsequent protein purification.
Product Description
| 22 ml + 22 ml (for 200 ml sample) – NAR911 | 22 ml NAR Reagent 1 and 22 ml NAR Reagent 2, enough to treat 200 ml of cell lysate or protein solution |
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Specifications
| Components | 22 ml NAR Reagent 1, 22 ml NAR Reagent 2 |
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| Capacity | 200 ml of cell lysate or protein solution |
| Residual nucleic acid | Below 250 pg/ml |
| Final salt | 0.45 M NaCl |
| Catalog # | NAR911 |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped cold (not frozen). |
Protocol Outline
- Keep all solutions at 0–4°C. Mix one volume of NAR Reagent 1 with 9 volumes of cell suspension (then lyse) or protein solution (then incubate on ice for 10 min).
- Remove insoluble material or precipitate by centrifugation at 6000 x g for 20 min at 4°C.
- Add one volume of NAR Reagent 2, incubate on ice for 5 min and remove the precipitate by centrifugation at 6000 x g for 20 min at 4°C.
- Continue with protein purification; for ion exchange chromatography, dialyze against a low-salt buffer first.
The ProFoldin protocol “Nucleic Acid Removal” is linked under Documents.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this Nucleic Acid Removal Kit — including larger pack sizes or bulk orders. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
