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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NUP50 was used as the immunogen for the NUP50 antibody. |
| Isotype | |
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Overview
NUP50 Antibody / Nucleoporin 50 is a anti-NUP50 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: NUP50
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF
Biological background
Nuclear pore complex protein Nup50 belongs to the FG (phenylalanine-glycine) repeat�containing family of nucleoporins that mediate selective permeability within the nuclear pore. It localizes to the nuclear basket region of the NPC, where it acts as a cofactor in cargo disassembly and receptor recycling. The NUP50 antibody supports visualization of its distribution along the inner nuclear envelope and nucleoplasmic face, highlighting its mobility during interphase and mitosis. Its high turnover reflects an adaptable role in transport regulation and chromatin organization.
Beyond its role in nuclear transport, Nuclear pore complex protein Nup50 has been implicated in gene regulation. It interacts with chromatin and transcription factors, suggesting a link between nuclear import and transcriptional control. The NUP50 antibody facilitates studies of this regulatory function, allowing researchers to explore how Nup50 coordinates transport with nuclear gene expression. Nup50 depletion alters transcriptional activity and nucleoplasmic organization, leading to cell-cycle arrest and reduced proliferation.
Defects in NUP50 expression have been associated with neurodevelopmental and neuromuscular disorders. Mutations or reduced expression may impair nuclear transport of essential regulatory proteins. The NUP50 antibody supports investigations into these pathologies, enabling assessment of expression and localization in neuronal and muscle tissues. It also serves as a useful marker for nuclear pore integrity in aging and neurodegenerative models.
The NUP50 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing characteristic nuclear-rim and nucleoplasmic staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
