| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
NR4A1, also known as Nur77, TR3, and NGFIB, is an orphan member of the NR4A subfamily of nuclear receptors. It binds as a monomer or homodimer to the NGF1B response element (NurRE/NBRE) in target gene promoters in a ligand-independent manner, and can also heterodimerize with COUP-TF and the retinoid X receptor to mediate transcription in response to retinoids. NR4A1 is an immediate-early gene induced by diverse stimuli and plays key roles in inflammatory responses in macrophages, T cell function, apoptosis, and metabolic regulation. Its activity is implicated in melanoma, colorectal cancer, and breast cancer, making it an actively studied target in immunology and oncology research.
Product Description & Applications
The NurRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of NR4A1 (Nur77) transcriptional activity in mammalian cells. The construct places a reporter gene downstream of tandem repeats of consensus NR4A1 DNA-binding elements, so reporter signal reflects endogenous NurRE-driven transcription. Stable lentiviral integration enables generation of polyclonal reporter cell lines with readout by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study NR4A1 signaling in inflammation, T cell biology, apoptosis, and cancer, and to screen modulators of NR4A1 activity. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NR4A1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
