| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
OCT4, encoded by POU5F1, is a POU-domain transcription factor and a master regulator of pluripotency. It is highly expressed in preimplantation blastocysts, embryonic stem cells, and other pluripotent cells, where it is essential for maintaining the undifferentiated state. OCT4 binds octamer DNA motifs, including palindromic OCT recognition elements as a homodimer, and cooperates with SOX2, NANOG, and the broader core regulatory network to control protein-coding genes and noncoding RNAs that sustain self-renewal. OCT4 is also one of the classic factors used to reprogram somatic cells into induced pluripotent stem cells, making it a central target in stem cell, developmental, and epigenetics research.
Product Description & Applications
The OCT4 Reporter Lentivirus is a transcription-factor reporter system for detecting OCT4-mediated transcriptional activity in mammalian cells. The construct uses tandem repeats of palindromic OCT recognition elements that report OCT4 homodimer activity, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on pluripotency, self-renewal, and cellular reprogramming.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the OCT4 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
