| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C16H15ClN4O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
OD36 is a RIPK2 inhibitor with an IC50 of 5.3 nM. It is a macrocyclic inhibitor with potent binding to the ALK2 kinase ATP pocket and shows ALK2-directed activity with a KD of 37 nM[1][2]. It is supplied as a white to off-white solid (C16H15ClN4O2, MW 330.77) at 99.33% purity.
Physical & Chemical Properties
| CAS Number | 1638644-62-8 |
|---|---|
| Molecular Formula | C16H15ClN4O2 |
| Molecular Weight | 330.77 g/mol |
| Purity | 99.33% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | ClC1=CC(C2=C3N/C(C=CN3N=C2)=N/CCOCCO4)=CC4=C1 |
| Target | RIPK2, ACVR1, ALK2 R206H |
| Signaling Pathway | Apoptosis; TGF-beta/Smad |
| Solubility | In Vitro: DMSO: 33.33 mg/mL (100.76 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
[1][2]
|
RIPK2 5.3 nM (IC50) |
ACVR1 37 nM (Kd) |
ACVR1 47 nM (IC50) |
ALK2 R206H 22 nM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 33.33 mg/mL (100.76 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
OD36 additionally inhibits ALK2 and ALK2 R206H, with IC50s of 47 and 22 nM, respectively[1]. OD36 is active against ALK1, with a KD of 90 nM[2]. OD36 potently antagonizes mutant ALK2 signaling and osteogenic differentiation[2]. In KS483 cells, OD36 (0.1-1 μM; 24 h) efficiently inhibits p-Smad1/5 induced by BMP-6 (50 ng/mL)[2]. Preincubating endothelial colony-forming cells (ECFCs) from fibrodysplasia ossificans progressiva (FOP) with OD36 (0.5 μM) completely prevents activin A-triggered activation of Smad1/5 and of the gene targets ID-1 and ID-3[2].
Western Blot Analysis[2]
- Cell Line: KS483 cells
- Concentration: 0.1, 0.2, and 1 μM
- Incubation Time:
- Result: Inhibited BMP-6 induced p-Smad1/5.
In Vivo
In a mouse model of acute peritonitis, OD36 (6.25 mg/kg; i.p.; once) reduces inflammation[3].
| Animal Model | C57BL/6 mice, muramyl dipeptide (MDP)-induced model of peritonitis[3] |
|---|---|
| Dosage | 6.25 mg/kg |
| Administration | Intraperitoneal injection, 30 min prior to MDP |
| Result | Inhibited the recruitment of inflammatory cells to the peritoneum, specifically that of neutrophils, and, to a lesser extent, lymphocytes. Decreased RIPK2-specific genes as well as inflammatory cytokine and chemokine gene expression. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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