| Field | Specification |
|---|---|
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| Applications | |
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| Purity | |
| Form | Solid |
| Storage | |
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Compound Overview
ODN 2007 is a class B CpG ODN (oligodeoxynucleotide) that functions as a Toll-like receptor (TLR) ligand. It can serve as an immunomodulator and vaccine adjuvant, enhancing immune responses in mammals, fish, and humans, and its sequence is 5'-TCGTCGTTGTCGTTTTGTCGTT-3'[1][2][3]. It is supplied as a white to off-white solid (MW 6852.50) at 97.86% purity.
Physical & Chemical Properties
| CAS Number | 455348-63-7 |
|---|---|
| Molecular Weight | 6852.50 g/mol |
| Purity | 97.86% |
| Appearance | Solid |
| Color | White to off-white |
| Sequence | DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T) |
| Signaling Pathway | Immunology/Inflammation |
| Solubility | In Vitro: H2O: 20 mg/mL (2.92 mM; Requires sonication) |
| Storage | -20°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
[2]. Hua Chen, et al. CpG-ODN 2007 protects zebrafish (Danio rerio) against Vibrio vulnificus infection. Aquac Res. 2021; 52: 897-905.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| H2O | 20 mg/mL (2.92 mM) | requires sonication |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
If water is used as the stock solvent, dilute to the working solution and sterilize it through a 0.22 μm filter before use.
Data provided by the manufacturer.
In Vitro
ODN 2007 (1 or 5 μg/mL, 3-18 h) significantly induces interferon IFN-γ and IFN-β expression in chicken macrophages[1]. ODN 2007 (10 μg/mL, 0.5-12 h) can raise phosphorylation levels of ERK2 and AKT and stimulate NO production[3].
RT-PCR[1]
| Cell Line | MQ-NCSU cells (a chicken macrophage cell line) |
|---|---|
| Concentration | 1 μg/mL, 5 μg/mL |
| Incubation Time | 3 h, 12 h, 18 h |
| Result | Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose. Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point. |
Western Blot Analysis[3]
| Cell Line | HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line |
|---|---|
| Concentration | 10 μg/mL |
| Incubation Time | 0.5 h, 1 h, 3 h, 6 h, 12 h |
| Result | Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels. Stimulated an increase in the level of NO. |
In Vivo
A single intraperitoneal injection of ODN 2007 (1 μg) can enhance the immune response in zebrafish infected with Vibrio traumaticus FJ03-X2, by regulating the expression of immune system-related genes[2].
| Animal Model | Zebrafish infected with Vibrio traumaticus FJ03-X2[2] |
|---|---|
| Dosage | 1 μg |
| Administration | Intraperitoneal injection; once |
| Result | Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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