| Field | Specification |
|---|---|
| Alternative names | OGM; GPR68-IN-1; OGM-8345 |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C21H17N3OS |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Ogremorphin, also known as OGM, GPR68-IN-1 and OGM-8345, is an inhibitor of the G protein-coupled sensor GPR68 with anti-inflammatory and anti-tumor activities. It can inhibit the migration of human melanoma cells and induce ferroptosis in glioblastoma cells[1][2][3]. It is supplied as a yellow to orange solid (C21H17N3OS, MW 359.44) at 99.13% purity.
Physical & Chemical Properties
| CAS Number | 352563-21-4 |
|---|---|
| Molecular Formula | C21H17N3OS |
| Molecular Weight | 359.44 g/mol |
| Purity | 99.13% |
| Appearance | Solid |
| Color | Yellow to orange |
| SMILES | O=C1NC2=CC=C(CC)C=C2C13SC(C4=C5C=CC=CC5=CC=C4)=NN3 |
| Signaling Pathway | GPCR/G Protein; Apoptosis |
| Solubility | In Vitro: DMSO: 50 mg/mL (139.11 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
[1]. Karki P, et al. A Novel Group of Ogremorphin Inhibitors Targeting G Protein-coupled Sensor GPR68 Rescue Lipopolysaccharide or Acidosis-induced Lung Endothelial Dysfunction[M]//C108. MECHANISMS OF LUNG INFLAMMATION AND INFECTION. American Thoracic Society, 2024: A6846-A6846.
[2]. Williams C H, et al. Coupling Metastasis to pH-Sensing GPR68 Using a Novel Small Molecule Inhibitor[J]. BioRxiv, 2019: 612549.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (139.11 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 1.25 mg/mL (3.48 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 1.25 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (12.5 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Data provided by the manufacturer.
In Vitro
In human pulmonary artery endothelial cells, Ogremorphin (1-5 μM) can lower the release of inflammatory factors induced by lipopolysaccharide or acidosis and suppress inflammatory responses[1]. Ogremorphin (5 μM, 5 days) can block migration of human melanoma WM-115 cells[2]. In glioblastoma U87 cells, Ogremorphin (0-100 μM, 72 h) can induce ferroptosis and reduce tumor cell viability by promoting expression of iron death related markers and mediators[3]. In patient derived cell lines (PDX and neurospheres), Ogremorphin (0-100 μM) can lower survival rate, with LC50 values of 0.42-2.7 μM[3].
Assay of cell viability[3].
| Cell Line | U87 and U138 cell lines |
|---|---|
| Concentration | 0-100 μM |
| Incubation Time | 72 h |
| Result | Reduced the vitality of glioblastoma cells. |
RT-PCR[1].
- Cell Line: Human pulmonary arterial endothelial cells (HPAECs)
- Concentration: 1-5 μM
- Incubation Time:
- Result: Reduced the upregulation of inflammatory cytokine/chemokine genes TNF - α, ICAM-1, VCAM-1, IL-6, IL-8, IL-1 β, and CXCL5.
Assay of cell migration[2].
| Cell Line | WM115, A2050 and MEWO cell lines |
|---|---|
| Concentration | 5 μM |
| Incubation Time | 5 days |
| Result | Inhibited migration in WM115, A2050 and MEWO cell lines. |
In Vivo
In mice, Ogremorphin can ease pulmonary endothelial dysfunction, pulmonary inflammation, and vascular leakage following intratracheal injection of LPS or acidosis[1]. In zebrafish embryos, Ogremorphin (10 μM) can lead to abnormal melanocyte pigmentation and developmental disorders[3].
| Animal Model | Zebrafish[3]. |
|---|---|
| Dosage | 10 μM |
| Administration | Added to a 96 well microtiter plate (5 embryos/well) arranged with zebrafish fertilized eggs |
| Result | Induced phenotypes encompassing a wavy notochord, abnormal pigmentation, craniofacial defects, ventral curvature, and a shortened body axis. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Pharmacological Activation of GPR68 Attenuates Ferroptosis in Spinal Cord Ischemia/Reperfusion Injury Through PI3K/Akt-Mediated Nrf2 Antioxidant Pathway. Inflammation 2025 Jun 18. PMID: 40528120
Targeting GPR68 Alleviates Inflammation and Lipid Accumulation in Metabolic Dysfunction-Associated Steatohepatitis. Biology (Basel) 2026 Jan 26;15(3):233. PMID: 41677704
