| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 6-(Benzylamino)-2-(2-hydroxyethylamino)-9-methylpurine.
- CAS number: 101622-51-9
- Molecular formula: C15H18N6O.
- Purity: >99%
- Concentration: 20-200 µM.
- Form: Lyophilized powder.
- Solubility: DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: Cyclin-Dependent Kinase (CDK)
- Source: Synthetic
- Activity: Olomoucine selectively inhibits cdc2/cyclin B (IC50 = 7 μM), cdk2/cyclin A (IC50 = 7 μM), cdk2/cyclin E (IC50 = 7 μM), cdk/p35 kinase (IC50 = 3 μM) and ERK1/MAP kinase at higher concentrations (IC50 = 25 μM)1. It also prevents NGF-mediated survival of neuronal cells2.
Scientific background
Olomoucine is a cell-permeable purine protein kinase inhibitor, found to be highly specific for the cell-cycle regulating p34cdc2/cyclin B, p33cdk2/cyclin A and p33cdk2/cyclin E kinases, the brain p33cdk5/p35 kinase and the ERK1/2 MAP- kinase (IC50=7-25 µM).1 Olomoucine behaves as a competitive inhibitor for ATP and as a non-competitive inhibitor for histone H1 binding in CDKs kinases.1-5Olomoucine induces a dose-dependent inhibition of proliferation rate and the cell cycle progression in many human cancer cells in culture (EC50 = 40-90 µM). Olomoucine, at 1-100 µM, slowdown or arrest KB 3-1 cells in G1/S and G2/M phase and decreased the DNA synthesis of rat brain cortex.6-11Olomoucine also induces apoptosis in maturing cerebellar granule neurons,11 NGF induced neuronal differentiation of PC12 cells in vitro12 and in NE cells in vivo.13
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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