| Field | Specification |
|---|---|
| Mfr No | |
| Form | 5X Mastermix |
| Product Type | |
| Storage |
OneScript® Hot 5X RT MasterMix is a convenient, ready-to-use formulation designed for efficient and reliable first-strand cDNA synthesis. This optimized mix simplifies the workflow while ensuring exceptional reproducibility and performance. It contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a precisely balanced combination of Oligo(dT)s and Random Primers. The OneScript® Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for improved cDNA yield and integrity. The resulting high-quality cDNA is ideal for a wide range of downstream molecular biology applications.
Product Features:
- Easy one-step setup reduces pipetting and sample handling
- High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
- Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
- Also includes dNTPs, Oligo (dT)s, and random primers
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| OneScript® Hot 5X RT MasterMix | 100 rxn (400 µl) |
| Nuclease-Free H2O | 1.0 ml |
 |
ISO 13485:2016 MDSAP Certified |
|
This product is equivalent to RScript Hot 5X RT MasterMix (Cat. No. G590EU). |
| Component | Quantity |
|---|---|
| OneScript® Hot 5X RT MasterMix | 100 rxn (400 µl) |
| Nuclease-Free H2O | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RT Mastermix |
| Format | 5X Mastermix |
| Storage Conditions | Store at -20°C. |
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
Store the synthesized first-strand cDNA at -20°C for long-term use.
Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield: Check RNA integrity with gel electrophoresis or a BioAnalyzer, aiming for a minimum A260/A280 ratio of 1.7 or a RIN above 8. Clean the RNA using ethanol precipitation or lithium chloride to remove contaminants. Purify the RNA with extraction kits or phenol/chloroform to remove proteins. Increase RNA input, especially for low-abundance samples.
We recommend using 1 ng to 2 μg of RNA per reaction.
Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
Can’t find what you’re looking for, or need a format that isn’t listed? BioHippo specializes in sourcing life science reagents across a broad supplier network and can help you locate the right product for your research — even if it isn’t currently in our catalog.
▶ Contact Us for Sourcing
If you need a specific size, concentration, format, or specification not listed here, reach out to our team. We regularly source products on behalf of researchers and can advise on the closest available alternative or arrange a custom inquiry.
Email us at support@biohippo.com and we will respond within one business day.
▶ Custom & Specialized Product Needs
For customized reagent formats — including bulk quantities, custom concentrations, or specialized formulations — visit our Research Services page to explore available services and initiate a request. Our team can connect you with the right resources for your project needs.
ⓘ BioHippo is a marketplace and life science sourcing partner. Contact us at support@biohippo.com to discuss your project.
