Ononetin

Product details

• Chemical name: 1-(2,4-dihydroxyphenyl)-2-(4-methoxyphenyl)ethanone.
• CAS number: 487-49-0
• Molecular formula: C15H14O4.
• Purity: >98%
• Concentration: 10-100 µM.
• Form: Lyophilized powder.
• Solubility: Soluble in DMSO and ethanol.Centrifuge all products before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: TRPM3, TRPA1 channels
• Source: Synthetic
• Activity: Ononetin is a deoxybenzoin, a potent blocker of TRPM3 channels (IC50 = 0.3 µM), also shown to activate TRPA1 channels at 10-100 µM1.

Scientific background

Ononetin is a deoxybenzoin that acts as a potent TRPM3 channel blocker with an IC50 of 0.3 µM and a TRPA1 channel activator between 10-100 µM. The compound binds at an extracellularly accessible binding site of the channel1.TRPM3, the third member of the melastatin-related TRPM channel subfamily, is a Ca2+-permeable non-selective cation channel expressed highly in the brain, spinal cord, sensory neurons, pituitary, kidney, eye, testis and adipose tissue. TRPM3 acts as a volume-regulated ion channel involved in the modulation of insulin release in pancreatic islets2.TRPA1, transient receptor potential ankyrin 1, is a non-selective cation channel expressed in the small neurons of the dorsal root and trigeminal ganglia. It plays an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds3.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.

• Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
• Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
• Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
• QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
• Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity

To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).

Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).