| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Description
OpenCRISPR-1 is a gene editing tool designed de novo using artificial intelligence technology, with a Cas9-like protein as its core component. This protein has undergone extensive sequence re-engineering, featuring up to 400 mutations compared to the widely used SpCas9, and nearly 200 distinct differences from any known natural CRISPR-associated protein. Despite these extensive modifications, the product retains gene editing efficiency and target specificity comparable to, or even superior than, SpCas9.
Features
Protein purity: ≥95%;
Bacterial endotoxin: <10 EU/mg;
Escherichia coli host cell DNA: <3 ng/mL;
Escherichia coli host cell protein: <0.1 ppm;
Mycoplasma contamination test: negative.
No residual nuclease, nicking enzyme, RNAase, or phosphatase (<0.0001 U);
Glycerol Content: Contains Glycerol
Specifications
|
Item No. |
14703ES60 / 14703ES80 |
|
Size |
100 μg / 1 mg |
|
Expression Host |
E.coli |
|
Concentration |
10 mg/mL |
|
Molecular Weight |
163.5 kDa |
|
Purity |
≥95% |
Components
|
Components No. |
Name |
14703ES60 |
14703ES80 |
|
14703-A |
OpenCRISPR-1 (10 mg/mL) |
10 μL |
100 μL |
|
14703-B |
10× Cas9 Nuclease Reaction Buffer |
500 μL |
1 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Application
Genome editing based on CRISPR/Cas technology;
Genetic modification of cell and gene therapy products (e.g., hematopoietic stem cells, T cells);
In vitro screening of effective sgRNAs;
In vitro cleavage of target DNA.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Gene Editing applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Nuclease activity is expressed in µg/µL; cleavage efficiency is validated as the percentage of target DNA cut within 60 min at 37°C using a defined guide RNA and target plasmid substrate.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
On-target efficiency is primarily determined by guide RNA (gRNA) design, target accessibility within chromatin, and PAM sequence context. Off-target cleavage is influenced by sequence complementarity mismatches (particularly in the seed region proximal to the PAM), enzyme concentration, and delivery method. High-fidelity Cas9 variants with engineered positional charge substitutions significantly reduce off-target activity (>99% reduction) while maintaining on-target cleavage efficiency comparable to wild-type enzyme.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
