P. aeruginosa IspD Assay Kit Plus

SKU:BHT16300094
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Overview
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P. aeruginosa IspD Assay Kit Plus with enzyme, MEP and CTP substrates, pyrophosphatase and a phosphate detection dye, for measuring IspD activity in a 384-well plate format. Available in 100-assay and 500-assay sizes with reagent volumes scaled to match.
Assay Target P. aeruginosa IspD activity
Plate Format 384-well plate
Options selector
Catalog no. Size
ISPD100KP 100 assays
ISPD500KP 500 assays
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 assays, 500 assays
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Organism P. aeruginosa
Applications
  • Enzyme Activity
Assay type
  • Enzyme Activity Assay
Assay target P. aeruginosa IspD activity
Assay area Antibacterial Drug Discovery
Target type Cytidyltransferase
Plate format 384-well plate
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) ISPD100KP | ISPD500KP
Main SKU BHT16300094
Drug Target Assay Kit

This kit is for measuring the activity of P. aeruginosa IspD (methylerythritol phosphate cytidyltransferase). It supplies the enzyme together with its substrates MEP and CTP.

Pack Sizes

Both sizes include P. aeruginosa IspD and pyrophosphatase. The only difference is the number of assays: ISPD100KP provides reagents for 100 assays and ISPD500KP for 500 assays (384-well format), with component volumes scaled accordingly.

100 assays (ISPD100KP) 100 assays; enzyme included
500 assays (ISPD500KP) 500 assays; enzyme included

Kit Components

Component 100 assays (ISPD100KP) 500 assays (ISPD500KP)
10 x Buffer 400 µl 2 ml
100 x MEP 35 µl 170 µl
100 x CTP 35 µl 170 µl
100 x P. aeruginosa IspD (200 nM) 35 µl 170 µl
100 x Pyrophosphatase (PPase, 10 U/ml) 35 µl 170 µl
Phosphate detection dye 5 ml 25 ml

Specifications

Assay Target P. aeruginosa IspD activity
Target Type Cytidyltransferase
Organism P. aeruginosa
Assay Type Enzyme Activity Assay
Plate Format 384-well plate
Sizes 100 assays, 500 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol

A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The P. aeruginosa IspD Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures the activity of P. aeruginosa IspD using its substrates MEP and CTP. Pyrophosphatase and a phosphate detection dye in the kit turn the reaction into a phosphate readout.
Q.What is the difference between the options?
A.Both sizes include P. aeruginosa IspD and pyrophosphatase. The only difference is the number of assays: ISPD100KP provides reagents for 100 assays and ISPD500KP for 500 assays (384-well format), with component volumes scaled accordingly.
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The P. aeruginosa IspD Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this P. aeruginosa IspD activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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