| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Plate format | |
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| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
This kit is for measuring the activity of P. aeruginosa IspD (methylerythritol phosphate cytidyltransferase). It supplies the enzyme together with its substrates MEP and CTP.
Pack Sizes
Both sizes include P. aeruginosa IspD and pyrophosphatase. The only difference is the number of assays: ISPD100KP provides reagents for 100 assays and ISPD500KP for 500 assays (384-well format), with component volumes scaled accordingly.
| 100 assays (ISPD100KP) | 100 assays; enzyme included |
|---|---|
| 500 assays (ISPD500KP) | 500 assays; enzyme included |
Kit Components
| Component | 100 assays (ISPD100KP) | 500 assays (ISPD500KP) |
|---|---|---|
| 10 x Buffer | 400 µl | 2 ml |
| 100 x MEP | 35 µl | 170 µl |
| 100 x CTP | 35 µl | 170 µl |
| 100 x P. aeruginosa IspD (200 nM) | 35 µl | 170 µl |
| 100 x Pyrophosphatase (PPase, 10 U/ml) | 35 µl | 170 µl |
| Phosphate detection dye | 5 ml | 25 ml |
Specifications
| Assay Target | P. aeruginosa IspD activity |
|---|---|
| Target Type | Cytidyltransferase |
| Organism | P. aeruginosa |
| Assay Type | Enzyme Activity Assay |
| Plate Format | 384-well plate |
| Sizes | 100 assays, 500 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this P. aeruginosa IspD activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.