P388 cell

SKU:BHC11101434
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Overview
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P388 cell is a pre B cell cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Mouse lymphoma
Growth Properties Suspension
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Catalog no. Size
305226 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305226
Species Mouse
P388 is a murine lymphoid neoplasm cell line derived from a spontaneous lymphocytic leukemia in DBA/2 mice. It is commonly used in cancer research, particularly for studying leukemia and testing anti-cancer compounds. P388 cells grow in suspension and exhibit a doubling time of approximately 24 hours under optimal culture conditions. The cells are characterized by their rapid proliferation and high sensitivity to chemotherapeutic agents, making them a valuable tool for evaluating the efficacy of new cancer treatments. P388 cells express typical markers of lymphoid lineage, including surface immunoglobulins and various cell surface antigens associated with B-cells. Researchers often utilize this cell line in in vivo models by inoculating mice to study tumor growth, metastasis, and response to therapies. Additionally, the P388 cell line serves as a model for investigating the molecular mechanisms underlying leukemia, such as the role of specific oncogenes and tumor suppressor genes. Despite its widespread use, the P388 cell line has limitations, such as the lack of human relevance and potential genetic drift over extended culture periods. Therefore, researchers often complement studies involving P388 cells with other models to obtain a comprehensive understanding of leukemia biology and treatment responses.

SKU:BHC11101434

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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